A Nutraceutical for Cancer Prevention Made from Chamuangone Extract with Rice Bran Oil

21Oct. 2016

Summary

Garcinia cowa is an edible plant commonly known in Thai as “Chamuang”. We have recently isolated a new polyprenylated benzophenone, namely “chamuangone” from G. cowa leaves. Chamuangone possessed a significant cytotoxic activity against lung adenocarcinoma (SBC3 and A549) and leukemia (K562, and K562/ADM) cells with IC50 values of 6.5, 7.5, 3.8, and 2.2 µM, respectively, as well as antibacterial activity against Streptococcus pyogenes (MIC 7.8 µg/mL), S. viridans and Helicobacter pylori (MICs 15.6 µg/mL), and Staphylococcus aureus, Bacillus subtilis and Enterococcus sp. (MICs 31.2 µg/mL) [1-2]. Therefore, G. cowa leaf extracts containing chamuangone may be potentially used as a dietary supplement for prevention of cancer. Thus, a suitable extraction method as well as a standardization process for preparation of G. cowa leaf extracts containing chamuangone should be optimized in order to get a consistently high quality extracts that can be effectively used in nutraceutical applications. A method for quantitative analysis of chamuangone in G. cowa leaf extracts has been established using a reversed-phase HPLC. In addition, hexane was a suitable solvent for extraction of chamuangone from G. cowa leaves using a microwave assisted extraction [2]. However, the use of hexane as a solvent for extraction is restricted due to its toxicities. Therefore, the present studies have focused on investigating the use of vegetable oils as the alternative green solvents for extraction of chamuangone from G. cowa leaves [3-4]. A nutraceutical, soft gelatin capsule containing G. cowa leaf extract (800 mg) that extracted with rice bran oil has been prepared using a green extraction method. The extracts are standardized to contain chamuangone of 1.9 mg/mL. The rice bran oil extract of G. cowa leaves exhibited satisfactory anticancer activities against human colorectal adenocarcinoma, breast adenocarcinoma and lung adenocarcinoma cells with IC50 values of 12 - 16 µg/mL, and no toxicity to normal cells.

References

[1] Sakunpak A., Panichayupakaranant P. 2012. Food Chemistry 130: 826-831.

[2] Sakunpak A., Panichayupakaranant P. 2013. Panta Medica 79: 1231.

[3]  Panichayupakaranant P., Sae-Lim P. 2016. Patent Application No. 1601001173. March 2, 2016.

[4]  Sae-Lim P., Panichayupakaranant P. 2016. The 4nd Current Drug Development International Conference. June 1-3, 2016, Phuket, Thailand.

Pharkphoom Panichayupakaranant, Pirunrat Sae-Lim, Supreeya Yuenyongsawad
Phytomedicine and Pharmaceutical Biotechnology Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, 90112, Thailand
Natural Product Research Center of Excellence and Department of Microbiology,
Faculty of Science, Prince of Songkla University
E-mail: supayang.v@psu.ac.th
Source : http://rdo.psu.ac.th/


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